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takanari inoue  (Addgene inc)


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    Addgene inc takanari inoue
    Takanari Inoue, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inpp5e/bio_rxiv__2025__06__06__657340-125-5-22?v=Addgene+inc
    Average 93 stars, based on 4 article reviews
    takanari inoue - by Bioz Stars, 2026-06
    93/100 stars

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    NPHP5 URECs show ciliogenesis and ciliary composition defects. (a–c) Control (1-56NC) and NPHP5 URECs (2.05P1, 2.05P2) grown in ciliogenesis conditions fixed and stained for primary cilia (ARL13B, green) and basal bodies (γ- tubulin, red) markers (a). (b) Ciliogenesis and (c) cilium length were quantified as in . (b) n = 4 experiments; mixed linear-regression model with quasibinomial penalization: ∗∗∗ P < 0.001. (c) n = 3 experiments; unpaired 2-tailed t test: ∗∗∗∗ P < 0.0001; ∗ P < 0.05. (d) Ciliated control (1.56.NC) and NPHP5 (2.05P1) URECs were fixed and stained for basal body and axoneme (GT335, red) and for TMEM67 (green), which is a marker of the transition zone (arrows). (e) TMEM67 staining intensity at the transition zone was quantified as detailed in Methods. n = 2 experiments; unpaired 2-tailed t test: ∗ P < 0.05. (f) Ciliated control (1.56.NC) and NPHP5 (2.05P1) URECS were fixed and stained for basal body and axoneme (GT335, red) and for <t>INPP5E</t> (green). (g) Intensity of INPP5E staining in cilia was quantified as explained in Methods. n = 3 experiments; unpaired 2-tailed t test: ∗∗ P < 0.01, ∗∗∗ P < 0.001. Scale bars 10 μm. UREC, urinary renal epithelial cells.
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    NPHP5 URECs show ciliogenesis and ciliary composition defects. (a–c) Control (1-56NC) and NPHP5 URECs (2.05P1, 2.05P2) grown in ciliogenesis conditions fixed and stained for primary cilia (ARL13B, green) and basal bodies (γ- tubulin, red) markers (a). (b) Ciliogenesis and (c) cilium length were quantified as in . (b) n = 4 experiments; mixed linear-regression model with quasibinomial penalization: ∗∗∗ P < 0.001. (c) n = 3 experiments; unpaired 2-tailed t test: ∗∗∗∗ P < 0.0001; ∗ P < 0.05. (d) Ciliated control (1.56.NC) and NPHP5 (2.05P1) URECs were fixed and stained for basal body and axoneme (GT335, red) and for TMEM67 (green), which is a marker of the transition zone (arrows). (e) TMEM67 staining intensity at the transition zone was quantified as detailed in Methods. n = 2 experiments; unpaired 2-tailed t test: ∗ P < 0.05. (f) Ciliated control (1.56.NC) and NPHP5 (2.05P1) URECS were fixed and stained for basal body and axoneme (GT335, red) and for <t>INPP5E</t> (green). (g) Intensity of INPP5E staining in cilia was quantified as explained in Methods. n = 3 experiments; unpaired 2-tailed t test: ∗∗ P < 0.01, ∗∗∗ P < 0.001. Scale bars 10 μm. UREC, urinary renal epithelial cells.
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    NPHP5 URECs show ciliogenesis and ciliary composition defects. (a–c) Control (1-56NC) and NPHP5 URECs (2.05P1, 2.05P2) grown in ciliogenesis conditions fixed and stained for primary cilia (ARL13B, green) and basal bodies (γ- tubulin, red) markers (a). (b) Ciliogenesis and (c) cilium length were quantified as in . (b) n = 4 experiments; mixed linear-regression model with quasibinomial penalization: ∗∗∗ P < 0.001. (c) n = 3 experiments; unpaired 2-tailed t test: ∗∗∗∗ P < 0.0001; ∗ P < 0.05. (d) Ciliated control (1.56.NC) and NPHP5 (2.05P1) URECs were fixed and stained for basal body and axoneme (GT335, red) and for TMEM67 (green), which is a marker of the transition zone (arrows). (e) TMEM67 staining intensity at the transition zone was quantified as detailed in Methods. n = 2 experiments; unpaired 2-tailed t test: ∗ P < 0.05. (f) Ciliated control (1.56.NC) and NPHP5 (2.05P1) URECS were fixed and stained for basal body and axoneme (GT335, red) and for INPP5E (green). (g) Intensity of INPP5E staining in cilia was quantified as explained in Methods. n = 3 experiments; unpaired 2-tailed t test: ∗∗ P < 0.01, ∗∗∗ P < 0.001. Scale bars 10 μm. UREC, urinary renal epithelial cells.

    Journal: Kidney International Reports

    Article Title: Prostaglandin Analogs and Eupatilin as Treatments for Nephronophthisis

    doi: 10.1016/j.ekir.2025.04.060

    Figure Lengend Snippet: NPHP5 URECs show ciliogenesis and ciliary composition defects. (a–c) Control (1-56NC) and NPHP5 URECs (2.05P1, 2.05P2) grown in ciliogenesis conditions fixed and stained for primary cilia (ARL13B, green) and basal bodies (γ- tubulin, red) markers (a). (b) Ciliogenesis and (c) cilium length were quantified as in . (b) n = 4 experiments; mixed linear-regression model with quasibinomial penalization: ∗∗∗ P < 0.001. (c) n = 3 experiments; unpaired 2-tailed t test: ∗∗∗∗ P < 0.0001; ∗ P < 0.05. (d) Ciliated control (1.56.NC) and NPHP5 (2.05P1) URECs were fixed and stained for basal body and axoneme (GT335, red) and for TMEM67 (green), which is a marker of the transition zone (arrows). (e) TMEM67 staining intensity at the transition zone was quantified as detailed in Methods. n = 2 experiments; unpaired 2-tailed t test: ∗ P < 0.05. (f) Ciliated control (1.56.NC) and NPHP5 (2.05P1) URECS were fixed and stained for basal body and axoneme (GT335, red) and for INPP5E (green). (g) Intensity of INPP5E staining in cilia was quantified as explained in Methods. n = 3 experiments; unpaired 2-tailed t test: ∗∗ P < 0.01, ∗∗∗ P < 0.001. Scale bars 10 μm. UREC, urinary renal epithelial cells.

    Article Snippet: The following antibodies were used: acetyl-a tubulin mouse monoclonal (1/300; Sigma, T6793), ADCY3 rabbit polyclonal (1/100e; Invitrogen, PA5-35382), ARL13B rabbit polyclonal (1/800e; ProteinTech, Thermo Fisher Scientific, 17711-1-AP), ARL13B mouse monoclonal (1/100e; ABCAM, Paris, France, ab136648), INPP5E rabbit polyclonal (1/200e; Proteintech, 17797-1-AP), γ-Tubulin mouse monoclonal (1/5000e; Sigma, T6557), GT335 mouse monoclonal (1/5000e; Adipogen, Coger, France, AG-20B-0020-C100), PH3 rabbit polyclonal (1/200; Cell Signaling Technology, Ozyme, Saint-Cyr L'Ecole, 3377S), NPHP4 rabbit polyclonal (1/100e; BiCell scientific, München, Germany, 90004), and NPHP11 rabbit polyclonal (1/100e; BiCell Scientific, 90103).

    Techniques: Control, Staining, Marker